Bioconversion of a Lignocellulosic Hydrolysate to Single Cell Oil for Biofuel Production in a Cost-Efficient Fermentation Process

Cutaneotrichosporon oleaginosus is a highly efficient single cell oil producer, which in addition to hexoses and pentoses can metabolize organic acids. In this study, fed-batch cultivation with consumption-based acetic acid feeding was further developed integrate the transformation of an industrial paper mill lignocellulosic hydrolysate (LCH) into yeast oil. Employing pentose-rich LCH as carbon source instead glucose significantly improved both biomass formation lipid titer, reaching 55.73 ± 5.20 g/L 42.1 1.7 (75.5% per biomass), respectively. This hybrid approach using one process optimized increase share bioavailable from combination continuous feeding. Finally, techno-economic analysis revealed 26% cost reduction when commercial glucose. summary, we leading holistic valorizing waste by converting it oleochemicals.